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KMID : 1005219940090030229
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Journal of Embryo Transfer
1994 Volume.9 No. 3 p.229 ~ p.234
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Production of a Normal Calf from Bovine Embryo Microinjected with Human Growth Hormone Gene
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손동수
김선정/김일화/서국현/이광원/상병돈/박무균/이철상/한용만
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Abstract
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This experiment was carried out to develop the model system for mass production of biomedical and nutritional proteins (human proteins) through mamraary gland of the transgenic cattle produced by gene manipulation and embryological technologies. Human growth hormone gene fused with rat β-casein gene promoter was microinjected into pronuclei of one cell bovine embryos produced by in vitro fertilization. After microinjection, embryos were cultured in vitro for 6 or 7 days. Twenty embryos reaching to blastocysts were transferred to 10 beef recipients, each receiving two embryos. Recipients were diagnosed for pregnancy by rectal palpation at 76 days after embryo transfer. One of them was pregnant to term and produced a female calf weighing 21 kg at 280 days following embryo transfer. DNA was extracted from umbilical cord tissue and blood of calf born for confirming gene insertion. As determined by Southern hybridization, the transgene was not found.
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